DNA reassociation kinetics and development of microsatellite libraries in black spruce (Picea mariana) and red spruce (Picea rubens) by Stefanie Wyss

Cover of: DNA reassociation kinetics and development of microsatellite libraries in black spruce (Picea mariana) and red spruce (Picea rubens) | Stefanie Wyss

Published by Laurentian University, School of Graduate in Sudbury, Ont .

Written in English

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Book details

Statementby Stefanie Wyss.
SeriesCanadian theses = Thèses canadiennes
The Physical Object
Paginationxi, 148 l. :
Number of Pages148
ID Numbers
Open LibraryOL22258305M
ISBN 109780494201930
OCLC/WorldCa298135038

Download DNA reassociation kinetics and development of microsatellite libraries in black spruce (Picea mariana) and red spruce (Picea rubens)

Microsatellite DNA/simple-sequence-repeat (SSR) loci were identified, isolated and characterized in white spruce (Picea glauca) by screening both a non-en Inheritance and linkage of polymorphic SSR loci were determined in F1 progeny of four controlled by: BAC clone sequencing and annotation.

As part of the first steps for the development of resources for complete genome sequencing and analysis of the tick R. microplus, a BAC library containing large insert sizes (mean insert size ≥ kbp) was library consists of 46, clones, providing ~ X coverage of the R.

microplus acquire basic knowledge about the genome Cited by: The development of microsatellite markers can be based on DNA sequence information deposited in databases, or it could be based on the screening of genomic DNA libraries specifically constructed for the discovery of repeated sequences in the by: Principal findings: A microsatellite-enriched library was constructed from genomic AFLP DNA fragments of black spruce.

The development of microsatellite markers can be based on DNA sequence information deposited in databases, or it could be based on the screening of genomic DNA libraries specifically constructed for the discovery of repeated sequences in the by:   Development of Microsatellite Markers from Fungal DNA Based on Shotgun Pyrosequencing.

Abstract. Traditional methods for the isolation and identification of fungal ­microsatellite markers mostly rely on construction of microsatellite-enriched DNA libraries and Sanger sequencing of clones from these : Shaobin Zhong.

DNA Sequencing and Microsatellite/AFLP Analysis. NEW USERS To use the GTAC you will need to meet with the laboratory director for a brief interview about your project and experience, register (so we have contact info. and a billing address), and then set up a time for an orientation to the lab (where things are, approved protocols, safety training, etc.).

Introduction. Microsatellite DNA loci have become important sources of genetic information for a variety of purposes (Goldstein and Schlotterer, see Chapter 14). To amplify microsatellite loci by polymerase chain reaction (PCR), primers must be developed from the DNA that flanks specific microsatellite repeats.

Repetitive DNA Sequences. A stretch of DNA sequence often repeats several times in the total DNA of a cell. For example, the following DNA sequence is just a small part oftelomerelocated at the ends of each human chromosome: An entire telomere, about 15 kb, is constituted by thousands of the repeated sequence "GGGTTA".

DNA reassociation kineticsis used to classify the repetitive DNA sequences.5/5(1). Finally, the last step in a PCR cycle is the polymerase extension step during which the DNA polymerase is producing a complementary copy of the target DNA strand starting from the PCR primer sequence.

The usual temperature of this step is 72oC, considered to be a good optimum temperature for thermal-stable polymerases. We have developed a near-saturated and complete genetic linkage map of black spruce using a three-generation outbred pedigree and amplified fragment length polymorphism (AFLP), selectively amplified microsatellite polymorphic loci (SAMPL), expressed sequence tag polymorphism (ESTP), and microsatellite (mostly cDNA based) by: Jingou Tong, Dan Wang and Lei Cheng (January 1st ).

Development of Microsatellite Markers by Data Mining from DNA Sequences, Data Mining and Knowledge Discovery in Real Life Applications, Julio Ponce and Adem Karahoca, IntechOpen, DOI: / Available from:Cited by: 1. ABSTRACT. The objective of this work was to develop new microsatellite markers in common bean.

Ninety nine new microsatelitte loci were developed from a microsatellite enriched library for (CT) 8 and (GT) 8 motifs, from CAL line. The majority of microsatellite sequences (51%) was related to.

— The organization of the DNA sequences in the N. tabacum genome was determined from the reassociation kinetics of long DNA fragments as well as S1 nuclease resistance and hyperchromicity. CD Genomics has extensive experience in providing support for the selection and design of microsatellite markers for a wide range of plant and animal species.

We offer a full service which includes assistance in designing and ordering fluorescently labeled primer pairs for the microsatellite markers of your choice.

The DNA samples were digested with AFAI and enriched using (CT)8 and (GT)8 biotinylated microsatellite probes for the dinucleotide library and (ATC)8 and (CCT)8 for the trinucleotide library.

The clones obtained were sequenced and the sequences were evaluated with the Microsat program, which removes parts of the vector and the adapters and Author: Camila Campos Mantello, Fernando Issamu Suzuki, Livia Moura Souza, Paulo de Souza Gonçalves, Anete P. The development of microsatellite markers in Passiflora has been based on the construction of genomic libraries enriched for simple sequence repeats [17,18,19, 23, 30,31,32].

This is an effective but time and labor consuming technique that can lead to microsatellite discovery and marker by: 7. We report the first genetic linkage map of Heliconius erato, a species that shows remarkable variation in its warningly colored wing patterns.

Cited by: Isolation and Development of Microsatellite DNA Markers. We have developed highly informative single-locus genomic microsatellite DNA markers in black spruce and red spruce using an AFLP-based microsatellite enrichment and isolation method.

This is the first report for microsatellite marker development in red spruce. Survey of microsatellite DNA in pine C.S. Echt and P.

MaylMarquardt Abstract: A large insert genomic library from eastern white pine (Pinus strobus) was probed for the microsatellite motifs (AC), and (AG), all 10 trinucleotide motifs, and 22 of the 33 possible tetranucleotide motifs.

Cited by: Microsatellite amplification was done using three of the primer sets used previously and seven proprietary markers from Perkin Elmer, and the products of DNA amplification by the polymerase chain.

Travis C. Glenn, Nancy A. Schable, in Methods in Enzymology, Introduction. Microsatellite DNA loci have become important sources of genetic information for a variety of purposes (Goldstein and Schlotterer, see Chapter 14).To amplify microsatellite loci by polymerase chain reaction (PCR), primers must be developed from the DNA that flanks specific microsatellite repeats.

Microsatellite data analysis version (T. Fér, F. Kolář) 1. Basic analysis of microsatellite data using MSA • First load the required libraries – i.e. add the following text to your script and Run library (adegenet) library (adegraphics) library (pegas)File Size: KB.

Microsatellite variation within and among North American lineages of Phragmites australis. Microsatellite DNA analysis of spatial and temporal population structuring of Li‐Rui Zhang, Elena Conti, Barbara Keller and Michael D.

Nowak, Development of 12 polymorphic microsatellite loci in the high alpine perennial. That is, certain microsatellite alleles are associated (through genetic linkage) with certain mutations in coding regions of the DNA that can cause a variety of medical disorders.

They have also become the primary marker for DNA testing in forensics (court) contexts -- both for human and wildlife cases (e.g., Evett and Weir, ). Whether you've loved the book or not, if you give your honest and detailed thoughts then people will find new books that are right for them.

1 Life System Modeling and Simulation: International Conference on Life System Modeling, and Simulation, LSMSShanghai, China, SeptemberAuthors: Echt, C.S.; Vendramin, G.G.; Nelson, C.

D.; Marquardt, Paula E. Publication Year: Publication Series: Scientific Journal (JRNL) Source: Canadian Journal of Forest Research. () Abstract. Polymerase chain reaction (PCR) primer pairs for 21 simple sequence repeat (SSR) loci in Pinus strobus L, and 6 in Pinus radiata D.

Don were evaluated to determine whether SSR Cited by: microsatellite DNA A form of highly repetitive DNA consisting of large numbers of very short base sequences scattered throughout the eukaryote genome. Each consists of a short base sequence, generally 2–10 bp long, that is repeated a variable number of times in a tandem array at any given locus.

A fundamental characteristic of a species is its karyotype, a description of each chromosome. In spruce (Picea) and other conifer genera, chromosome identification is hindered by similarities in chromosome size and morphology within a species. Homeologous chromosomes of related species are also generally indistinguishable.

The focus of this Ph.D thesis is the development of a new technology in Author: Garth Robert Brown. Microsatellite markers are co-dominant, polymorphic DNA loci containing repeated nucleotide sequences, typically with 2 to 10 nucleotides per repeated unit. The number of nucleotides in the repeated unit is the same for the majority of the repeats within an individual microsatellite locus, but the number of repeats for a specific locus may.

Stem rust has been a serious disease of wheat, barley, oat and rye, as well as various important grasses including timothy, tall fescue and perennial ryegrass. The stem rust funguCited by: A microsatellite is a tract of repetitive DNA in which certain DNA motifs are repeated, typically 5–50 times.

Microsatellites occur at thousands of locations within an organism's genome. They have a higher mutation rate than other areas of DNA leading to high genetic diversity. Microsatellites are often referred to as short tandem repeats by forensic geneticists and in genetic genealogy, or as simple sequence.

Microsatellites, New Genetic Markers. A microsatellite is a stretch of DNA made of a two to four base-pair long sequence that is repeated in tandem - e.g.

a stretch of DNA that looks like this: CAGCAGCAGCAGCAGCAGCAG. Red pine (Pinus resinosa Ait.) is an ecologically and economically important forest tree species of northeastern North America and is considered one of the most genetically depauperate conifer species in the region.

We have isolated and characterized 13 nuclear microsatellite loci by screening a partial genomic library with di- tri- and tetranucleotide repeat oligonucleotide by: The development of an appropriate genetic conservation strategy for native Scots pine populations in European countries seems to be a very relevant priority.

Numerous nuclear microsatellite markers have already been described for different conifer species, for example, fir, larch, pine, and spruce (for review, see [9, 14, 19, 31, 33, 65–69]).Author: Justyna Anna Nowakowska.

Abstract. Aspergillus spp. have been the subject of numerous epidemiological studies. The most useful typing techniques are DNA based methods including the random amplified polymorphic DNA technique, microsatellite length polymorphisms, restriction fragment length polymorphism (RFLP) analysis using retrotransposon-like sequences as probes, and multilocus sequence by:   Detailed information of microsatellite DNA contains basic information of microsatellite DNA such as location, type, size and sequence, and primer sequences designed by Primer3 software.

In addition, corresponding genes or transcripts and associated biological functions are also provided if applicable (34) (Figure 2F).Cited by: Other articles where Microsatellite DNA is discussed: heredity: Repetitive DNA: Microsatellite DNA is composed of tandem repeats of two nucleotide pairs that are dispersed throughout the genome.

Minisatellite DNA, sometimes called variable number tandem repeats (VNTRs), is composed of blocks of longer repeats also dispersed throughout the genome.

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Spruce>Beech>Chestnut. The highest level of ecological organization is. The biosphere. Using microsatellite DNA, Douglas and Brunner concluded that populations of Coregonus found in the Alps were highly differentiated and should be managed as separate units.

TRUE.Microsatellite-Enhanced Genomic Library Construction Streptavidin-Biotin Bench Version SNX Linker Purpose: To construct and clone genomic libraries with increased proportions of inserts that contain tandem repeat arrays. Thus, a greater number of microsatellite repeat regions can be detected, sequenced and subsequently used to design species.

The company, DNA Print Genomics in Sarasota, Fla., starts with DNA from a customer's inner cheek. After comparing the sample's genetic markers with those in a data set collected from people around the world, the company estimates what percentage of the person's ancestry is African, East Asian, European, or Native American.

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